School of Veterinary Medicine

Comparative Pathology Lab

Molecular Diagnostics

PCR Technician

Polymerase Chain Reaction (PCR) technology is a large part of the diagnostic services offered at CPL. Rapid and specific screenings for viral and bacteriological pathogens are accomplished on our group of dedicated instruments, in areas specifically set aside for molecular diagnostics.  We accept culture media for contamination screening, fecal samples, liver, spleen, lung, kidney, lymph nodes and other organ tissues for analysis.

For facilities engaged in implant research, we can also assay cell culture preparations, embryonic stem cells (ESC) or other biologics that require screening before project use.  Environmental swabs or rodent cage filters in sterile saline may also be evaluated.

Organisms we commonly screen for are:

  • Helicobacter species (most often from fecal pellets or cecal tissue)
  • Mycoplasma species (most often on ESC and lung tissue)
  • Pneumocystis carinii (on tracheal washes and lung tissue)
  • Encephalitozoon cunniculi (on urine and CSF)
  • Mouse Parvo Virus & Mouse Hepatitis Virus (on feces, mesenteric lymph nodes and spleen)
  • Q-Fever in sheep / goats (on amniotic fluid, placenta tissue or buffy-coat preparations)
PCR Technician

For special molecular diagnostic procedures involving organisms not specifically mentioned, please feel free to call and inquire. We periodically develop assays for additional organisms, so our assay menus are evolving.

Thermalcyclers