Serological testing remains a very major component of a well-managed Rodent Health Surveillance program. Many university-based rodent biology programs subscribe to our services throughout the year, in order to ensure confidence in their colony health. Commercial biotech conducting research with mouse or rat models also routinely deliver samples for screening. Serology services use advanced, state-of-the-art Luminex multiplexed fluorometric bead-based immunoassay technology, in conjunction with conventional ELISA and IFA methods. These technologies support comprehensive screening panels for rodent viral and bacterial pathogens. The use of multiplexed serological testing systems are becoming the standard of the industry, and CPL is committed to those technology platforms.
In situations where facilities import new experimental or breeding animals into environmentally controlled areas, we provide capabilities to test live sentinel subjects or blood samples for a wide variety of significant pathogens. Combined with microscopic parasite examinations and molecular diagnostic PCR assays, we are able to perform comprehensive health monitoring that helps to “clear” new populations of subjects into existing clean colonies.
We have many levels of serological panels tailored to meet the needs of quarterly, semi-annual and annual sentinel monitoring programs. We have surveyed many clients and have designed our panels to include the most routinely requested profiles. CPL can help you achieve compliance to industry standard health certifications. Our staff is experienced, and can easily custom-fit individual etiologies into your testing regimen. Please inquire or review our service listing to see the many etiologies we assay for. For your convenience, that service listing also has an identification key that refers to the many abbreviations commonly used for rodent pathogens.
Since researchers may have some dificulty obtaining large sample volumes, here are some preferred generalized serum sample volumes for testing:
- For any single etiology serological testing, we need at least 30 uL of serum to perform the assay.
- For our comprehensive Level 1 panel, we prefer 100 uL serum.
- For our most comprehensive Level 2 panels, we prefer approximately 200 uL serum.
If you cannot obtain the preferred amount of serum, we can dilute your specimen to perform testing, but please call us to check on feasibility.
In order to preserve sample integrity, whole blood may be submitted instead of serum, if samples are sent via overnight priority mail. We will then centrifuge and aliquot the serum at our facility. Specimens are best preserved and transported refrigerated at 2-8° Centigrade. Ship specimens via overnight priority mail, using insulated containers with well-frozen ice packs.