Molecular Diagnostics / PCR

We offer comprehensive quantitative PCR and RT-PCR assays for viral, bacterial and metazoan pathogens of rodent, ruminant, and aquatic animals.

Assays can be developed as novel pathogens are discovered (Mouse Kidney Parvovirus or Murine Chapparvovirus (MuCPV)) or outbreaks occur.

 

Graph showing amplification cycles for Mouse Kidney Panvirus with three colored curves.

 

Test

 

Recommended Sample Type(s)

Rate code

Rodent pathogens   
 Corynebacterium bovisfur/skin swab, skin, environmental/cage swabB070
 Helicobacter (Species, h. hepaticus, h. bilis, h. rodentium, h. typhlonius)cecal wall, feces (up to five fecal pellets can be pooled), environmental swabB020
 Mycoplasma sp. (All species)cell culture, lung, oral swab, abscess swabB060
 Pseudomonas aeruginosacecal contents, feces, environmental swabB070
 Encephalitozoon cuniculifeces, kidneyB070
 Fur Mites (Myocoptes musculinis, Radfordia/Myobia sp.)fur swab, environmental/cage swabB090
 Pinworms (Aspiculuris tetraptera, Syphacia obvelata, Syphacia muris)feces, environmental/cage swabB070
 Pneumocystis carinii/murinalungB040
 Mouse hepatitis virus (MHV)feces, liverB130
 Mouse kidney parvovirus (MKPV)kidney, feces, urineB120
 Mouse norovirus (MNV)feces, environmental swabB080
 Mouse parvovirus 1-7 (MPV)mesenteric lymph node, feces, spleenB110
    
Zoonotic pathogens   
 Q fever (Coxiella burnettii)amniotic fluid, placenta, uterus, whole blood (EDTA tube)B050
Aquatic animal pathogens   
 Edwardsiella ictaluriwhole fish, headB070
 Ichthyophthirius multifiliiswhole fish, headB070
 Mycobacterium (Species, M. chelonae, H. haemophilum, M. marinum)whole fish, gutB070

Other assays and assay development services are available. Please inquire

 

Submissions

Please submit samples in a 1.5ml microcentrifuge tube when possible.  The seal-rite tubes are preferable since they have a tighter seal.  If submitting large samples, a 15 or a 50ml conical tube will suffice.  If submitting feces or swabs, up to 5 fecal pellets or 5 swabs can be pooled into one tube.  Ship samples to the lab priority overnight via FedEx or UPS.  Samples should be shipped in an insulated container with ice packs.  Please contact the lab with any questions.

Preferred Sample Collection Tubes

USA Scientific: Seal-Rite® 1.5 mL Microcentrifuge Tubes:  #1615-2500

Preferred sample collection tubes